Derived from purified and inactivated HTLV-I viral lysate, a purified HTLV-II viral lysate, and a recombinant HTLV-I p21E antigen, the Avioq HTLV-I/II Microelisa System is an enzyme-linked immunosorbent assay (ELISA) for the qualitative detection of antibodies to HTLV-I/II in human serum or plasma.
The Avioq HTLV-I/II Microelisa System is intended as a screen for donated blood and organs to prevent transmission of HTLV-I and HTLV-II to recipients of cellular blood components and as an aid in clinical diagnosis of HTLV-I or HTLV-II infection and related diseases.
Developed as a standard two-step indirect microelisa system assay, the Avioq HTLV-I/II Microelisa System contains coated 96-well microwell plates, color-coded, liquid negative and positive controls.
Kit Configuration and Principle of Test
The product is available in three kit configurations, the 192, 576, and 9600 test kits. Please refer to the 'Resource' tab for additional information, as well as the performance characteristics of this product. The Package Insert and Test Procedure Card are also available in the 'Resource' tab.
The Avioq HTLV-I/II Microelisa System is an enzyme-linked Immunosorbent assay in which the solid phase (Microwells) is coated with a purified HTLV-I viral lysate, a purified HTLV-II viral lysate, and a recombinant HTLV-I p21E antigen.
With the addition of a diluted test sample containing antibodies to either HTLV-I or HTLV-II, complexes are formed by the interaction of the antibodies in the sample and the solid phase antigens. Following incubation, the sample is aspirated and the well is washed with buffer. Subsequently, anti-human immunoglobulin (goat) conjugated with horseradish peroxidase (HRP) is added which binds the antibody-antigen complex during a second incubation.
Following a wash and incubation with TMB (Tetramethylbenzidine) substrate, a blue color is produced. The enzyme reaction is stopped by the addition of a sulfuric acid solution which changes the color to yellow. The amount of antibody present in the sample is proportional to color development.
Ease of Use
• Fast, convenient procedure: Total incubation time only 2 hours, 30 minutes
• No sample pre-dilution step
Time and Cost-Savings
• Excellent sensitivity and specificity in clinical trials providing reduced cost for confirmatory testing
• High correlation between initial and repeat duplicate results
• Direct dilution into microplate well
Confidence in Results
• Excellent performance characteristics to provide the “Right Answer Sooner”
• 99.95% specificity in random donor population seen in clinical trials (95% confidence interval of 99.89 to 99.98%)
• 100 % Sensitivity as seen in clinical trials (95% confidence interval of 99.97 to 100%)
• 96-well microplate with 8-well strips: Adaptable to automation and your testing volume
• 20 uL specimen volume for serum/plasma
• Consistent volumes for Wash Buffer, Conjugate, Substrate, and Stop solution
Avioq, Inc. is committed to provide microplate instruments to meet customer needs with our growing menu of immunodiagnostic products. The manual instrument options can meet the needs of low, medium, and high volume testing. Our microplate instrument offering includes options for ELISA Readers, Washers, Incubator, Pipettor/Dilutor, Computer with Data Analysis Software, and Printers.
Both the Avioq HIV-1 (IVD) and Avioq HTLV-I/II assays utilize 8-well strips with sample and reagent volumes that allow instrument flexibility. This includes compatibility with automated pipetting systems and full automated instruments (pipetting + plate processing).
Options provided by Avioq: these options have the validated Avioq assays that have been programmed into the specific instruments. The following instrumentation with the Avioq programming is available only through Avioq.
|AVIOQ PART NUMBER||INSTRUMENT TYPE||MODEL NUMBER||SALES FLYER|
|200173||Microelisa||BioTek ELx800||ELx800 SingleSheet|
|200174||Microelisa Reader||BioTek Elx808||ELx808 New|
|7560522||Test Absorbance Plate||BioTek|
|200170||Microelisa Strip Washer||BioTek ELx50||ELx50 Single Sheet|
|200172||Microelisa Full Plate Washer||BioTek ELx405||ELx405 Single Sheet|
|7100745||Dispense/Waste System||BioTek-for ELx405|
|201280||Pipettor/Dilutor||Hamilton 530b||Hamilton 530b|
|201180||Incubator (Forced Air)||10-140AE|
|201174||Data Analysis Software||BioTek Gen5 CL||Gen5CL|
|201179||Printer (Laser or Inkjet)||Dell or HP|
Note: For any instrument, the manual provided by the manufacturer should be reviewed for additional information regarding the following:
Installation and special requirements.
Operation principles, instructions, precautions, and hazards.
Manufacturer's specifications and performance capabilities.
Service and maintenance information.