Derived from purified and inactivated HIV-1 viral lysate proteins, the Avioq HIV-1 Microelisa System is an enzyme-linked immunosorbent assay (ELISA) for the qualitative detection of antibodies to HIV-1 (a leading causal agent of AIDS in the U.S.) in human specimens collected as serum, plasma, dried blood spots, or oral fluid specimens obtained with the OraSure® HIV-1 Oral Specimen Collection Device.
The Avioq HIV-1 Microelisa System is intended for use as an aid in diagnosis of infection with HIV-1. It is not intended for use in screening blood donors. Developed as a standard two-step indirect microelisa system assay, the Avioq HIV-1 Microelisa System contains coated 96-well microwell plates, color-coded, liquid calibrator and positive control, and a ready-to-use liquid substrate. The sample diluent contains sample addition monitoring to allow visualization of color change upon sample addition to microwells.
Kit Configuration and Principle of Test
The product is available in three kit configurations, the 384, 960, and 9600 test kits. Please refer to the 'Resource' tab for additional information, as well as the performance characteristics of this product. The Package Insert and Test Procedure Card are also available in the 'Resource' tab.
The Avioq HIV-1 Microelisa System uses HIV-1 antigens, including inactivated, purified HIV-1 viral lysate proteins, which are coated on the wells of microwell plates, for the detection of antibodies against HIV-1. Upon addition of a diluted test specimen containing antibodies to HIV-1 to a microwell, immune complexes are formed through the interaction between the anti- HIV in the specimen and the HIV antigens coated on the microwell.
Following incubation, the specimen is aspirated and the well is washed with buffer. Subsequently, anti-human immunoglobulin (goat) conjugated with horseradish peroxidase (HRP) is added which binds to the anti-HIV antigen complex during a second incubation. Following a wash and incubation with ABTS (2,2’-azino-di-(3-ethylbenzthiazoline-6-sulfonate)) substrate, a green color is produced. The enzyme reaction is stopped by the addition of a fluoride solution.
The amounts of antibodies to HIV present in the specimen are qualitatively proportional to color intensity. Because of the use of HIV-1 viral lysate as the primary antigens in the test, significant cross-reactivity with HIV-2 specimens was observed.
Ease of Use
• Fast, convenient procedure suitable for automation
• No sample pre-dilution step
• Color-coded calibrator and positive control
• Sample addition monitoring
Time and Cost-Savings
• Excellent sensitivity and specificity in clinical trials providing reduced cost for confirmatory testing
• High correlation between initial and repeat duplicate results
• High positive predictive value that saves unnecessary confirmatory testing
• Direct dilution into microplate well
Confidence in Results
• Color-coded reagents with sample addition monitoring
• Excellent performance characteristics to provide the “Right Answer Sooner”
• 96-well microplate with 8-well strips
• 10 uL specimen volume for serum/plasma
• Consistent volumes for Wash Buffer, Conjugate, Substrate, and Stop solutions
• Specimen diluent (Dilsim III) contains HIV-1 inactivator for safe microplate handling
Avioq, Inc. is committed to provide microplate instruments to meet customer needs with our growing menu of immunodiagnostic products. The manual instrument options can meet the needs of low, medium, and high volume testing.
Our microplate instrument offering includes options for ELISA Readers, Washers, Incubator, Pipettor/Dilutor, Computer with Data Analysis Software, and Printers.
Both the Avioq HIV-1 (IVD) and Avioq HTLV-I/II assays utilize 8-well strips with sample and reagent volumes that allow instrument flexibility. This includes compatibility with automated pipetting systems and full automated instruments (pipetting + plate processing).
Options provided by Avioq: these options have the validated Avioq assays that have been programmed into the specific instruments. The following instrumentation with the Avioq programming is available only through Avioq.
|AVIOQ PART NUMBER||INSTRUMENT TYPE||MODEL NUMBER||SALES FLYER|
|200173||Microelisa||BioTek ELx800||ELx800 SingleSheet|
|200174||Microelisa Reader||BioTek Elx808||ELx808 New|
|7560522||Test Absorbance Plate||BioTek|
|200170||Microelisa Strip Washer||BioTek ELx50||ELx50 Single Sheet|
|200172||Microelisa Full Plate Washer||BioTek ELx405||ELx405 Single Sheet|
|7100745||Dispense/Waste System||BioTek-for ELx405|
|201280||Pipettor/Dilutor||Hamilton 530b||Hamilton 530b|
|201180||Incubator (Forced Air)||10-140AE|
|201174||Data Analysis Software||BioTek Gen5 CL||Gen5CL|
|201179||Printer (Laser or Inkjet)||Dell or HP|
Note: For any instrument, the manual provided by the manufacturer should be reviewed for additional information regarding the following:
Installation and special requirements.
Operation principles, instructions, precautions, and hazards.
Manufacturer's specifications and performance capabilities.
Service and maintenance information.